OrangerV1, Main, Exploration, bibRecord, 000D19

Structural and Physiological Analyses of the Alkanesulphonate-Binding Protein (SsuA) of the Citrus Pathogen Xanthomonas citri

Identifieur interne : 000D19 ( Main/Exploration ); précédent : 000D18; suivant : 000D20

Structural and Physiological Analyses of the Alkanesulphonate-Binding Protein (SsuA) of the Citrus Pathogen Xanthomonas citri

Auteurs : Fabiano T Foli De Araújo [Brésil] ; Victor M. Bolanos-Garcia [Royaume-Uni] ; Cristiane T. Pereira [Brésil] ; Mario Sanches [Canada] ; Elisa E. Oshiro [Brésil] ; Rita C. C. Ferreira [Brésil] ; Dimitri Y. Chigardze [Royaume-Uni] ; João Alexandre Gonçalves Barbosa [Brésil] ; Luís Carlos De Souza Ferreira [Brésil] ; Celso E. Benedetti [Brésil] ; Tom L. Blundell [Royaume-Uni] ; Andrea Balan [Brésil]

Source :

PLoS ONE [ 1932-6203 ] ; 2013.

RBID : PMC:3839906

English descriptors

KwdEn :
- Alkanesulfonates (chemistry), Alkanesulfonates (metabolism), Amino Acid Sequence, Bacterial Proteins (chemistry), Bacterial Proteins (genetics), Bacterial Proteins (physiology), Binding Sites, Carrier Proteins (chemistry), Carrier Proteins (genetics), Carrier Proteins (physiology), Citrus sinensis (growth & development), Citrus sinensis (metabolism), Citrus sinensis (microbiology), Models, Molecular, Molecular Sequence Data, Plant Diseases (microbiology), Polysaccharides, Bacterial (metabolism), Sequence Alignment, Virulence, X-Ray Diffraction, Xanthomonas (genetics), Xanthomonas (metabolism), Xanthomonas (pathogenicity).
MESH :
- chemical , chemistry : Alkanesulfonates, Bacterial Proteins, Carrier Proteins.
- chemical , genetics : Bacterial Proteins, Carrier Proteins.
- chemical , metabolism : Alkanesulfonates, Polysaccharides, Bacterial.
- chemical , physiology : Bacterial Proteins, Carrier Proteins.
- genetics : Xanthomonas.
- growth & development : Citrus sinensis.
- metabolism : Citrus sinensis, Xanthomonas.
- microbiology : Citrus sinensis, Plant Diseases.
- pathogenicity : Xanthomonas.
- Amino Acid Sequence, Binding Sites, Models, Molecular, Molecular Sequence Data, Sequence Alignment, Virulence, X-Ray Diffraction.

Abstract

Background

The uptake of sulphur-containing compounds plays a pivotal role in the physiology of bacteria that live in aerobic soils where organosulfur compounds such as sulphonates and sulphate esters represent more than 95% of the available sulphur. Until now, no information has been available on the uptake of sulphonates by bacterial plant pathogens, particularly those of the Xanthomonas genus, which encompasses several pathogenic species. In the present study, we characterised the alkanesulphonate uptake system (Ssu) of Xanthomonas axonopodis pv. citri 306 strain (X. citri), the etiological agent of citrus canker.

Methodology/Principal Findings

A single operon-like gene cluster (ssuEDACB) that encodes both the sulphur uptake system and enzymes involved in desulphurisation was detected in the genomes of X. citri and of the closely related species. We characterised X. citri SsuA protein, a periplasmic alkanesulphonate-binding protein that, together with SsuC and SsuB, defines the alkanesulphonate uptake system. The crystal structure of SsuA bound to MOPS, MES and HEPES, which is herein described for the first time, provides evidence for the importance of a conserved dipole in sulphate group coordination, identifies specific amino acids interacting with the sulphate group and shows the presence of a rather large binding pocket that explains the rather wide range of molecules recognised by the protein. Isolation of an isogenic ssuA-knockout derivative of the X. citri 306 strain showed that disruption of alkanesulphonate uptake affects both xanthan gum production and generation of canker lesions in sweet orange leaves.

Conclusions/Significance

The present study unravels unique structural and functional features of the X. citri SsuA protein and provides the first experimental evidence that an ABC uptake system affects the virulence of this phytopathogen.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839906

DOI: 10.1371/journal.pone.0080083
PubMed: 24282519
PubMed Central: 3839906

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural and Physiological Analyses of the Alkanesulphonate-Binding Protein (SsuA) of the Citrus Pathogen <italic>Xanthomonas citri</italic>
</title>
<author><name sortKey="T Foli De Araujo, Fabiano" sort="T Foli De Araujo, Fabiano" uniqKey="T Foli De Araujo F" first="Fabiano" last="T Foli De Araújo">Fabiano T Foli De Araújo</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Bolanos Garcia, Victor M" sort="Bolanos Garcia, Victor M" uniqKey="Bolanos Garcia V" first="Victor M." last="Bolanos-Garcia">Victor M. Bolanos-Garcia</name>
<affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, University of Cambridge, Cambridge</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pereira, Cristiane T" sort="Pereira, Cristiane T" uniqKey="Pereira C" first="Cristiane T." last="Pereira">Cristiane T. Pereira</name>
<affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sanches, Mario" sort="Sanches, Mario" uniqKey="Sanches M" first="Mario" last="Sanches">Mario Sanches</name>
<affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Monte Sinai Hospital, Toronto, Ontario, Canada</addr-line>
</nlm:aff>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Monte Sinai Hospital, Toronto, Ontario</wicri:regionArea>
<wicri:noRegion>Ontario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Oshiro, Elisa E" sort="Oshiro, Elisa E" uniqKey="Oshiro E" first="Elisa E." last="Oshiro">Elisa E. Oshiro</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Ferreira, Rita C C" sort="Ferreira, Rita C C" uniqKey="Ferreira R" first="Rita C. C." last="Ferreira">Rita C. C. Ferreira</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Chigardze, Dimitri Y" sort="Chigardze, Dimitri Y" uniqKey="Chigardze D" first="Dimitri Y." last="Chigardze">Dimitri Y. Chigardze</name>
<affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, University of Cambridge, Cambridge</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Barbosa, Joao Alexandre Goncalves" sort="Barbosa, Joao Alexandre Goncalves" uniqKey="Barbosa J" first="João Alexandre Gonçalves" last="Barbosa">João Alexandre Gonçalves Barbosa</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Departamento de Genética, Universidade Católica de Brasília, Brasilia, Districto Federal, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Genética, Universidade Católica de Brasília, Brasilia, Districto Federal</wicri:regionArea>
<wicri:noRegion>Districto Federal</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="De Souza Ferreira, Luis Carlos" sort="De Souza Ferreira, Luis Carlos" uniqKey="De Souza Ferreira L" first="Luís Carlos" last="De Souza Ferreira">Luís Carlos De Souza Ferreira</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Benedetti, Celso E" sort="Benedetti, Celso E" uniqKey="Benedetti C" first="Celso E." last="Benedetti">Celso E. Benedetti</name>
<affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Blundell, Tom L" sort="Blundell, Tom L" uniqKey="Blundell T" first="Tom L." last="Blundell">Tom L. Blundell</name>
<affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, University of Cambridge, Cambridge</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Balan, Andrea" sort="Balan, Andrea" uniqKey="Balan A" first="Andrea" last="Balan">Andrea Balan</name>
<affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24282519</idno>
<idno type="pmc">3839906</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839906</idno>
<idno type="RBID">PMC:3839906</idno>
<idno type="doi">10.1371/journal.pone.0080083</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">001180</idno>
<idno type="wicri:Area/Pmc/Curation">001179</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000979</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">000411</idno>
<idno type="wicri:Area/PubMed/Curation">000411</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000411</idno>
<idno type="wicri:Area/Ncbi/Merge">001517</idno>
<idno type="wicri:Area/Ncbi/Curation">001517</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001517</idno>
<idno type="wicri:Area/Main/Merge">000D23</idno>
<idno type="wicri:Area/Main/Curation">000D19</idno>
<idno type="wicri:Area/Main/Exploration">000D19</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structural and Physiological Analyses of the Alkanesulphonate-Binding Protein (SsuA) of the Citrus Pathogen <italic>Xanthomonas citri</italic>
</title>
<author><name sortKey="T Foli De Araujo, Fabiano" sort="T Foli De Araujo, Fabiano" uniqKey="T Foli De Araujo F" first="Fabiano" last="T Foli De Araújo">Fabiano T Foli De Araújo</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Bolanos Garcia, Victor M" sort="Bolanos Garcia, Victor M" uniqKey="Bolanos Garcia V" first="Victor M." last="Bolanos-Garcia">Victor M. Bolanos-Garcia</name>
<affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, University of Cambridge, Cambridge</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pereira, Cristiane T" sort="Pereira, Cristiane T" uniqKey="Pereira C" first="Cristiane T." last="Pereira">Cristiane T. Pereira</name>
<affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sanches, Mario" sort="Sanches, Mario" uniqKey="Sanches M" first="Mario" last="Sanches">Mario Sanches</name>
<affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Monte Sinai Hospital, Toronto, Ontario, Canada</addr-line>
</nlm:aff>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Monte Sinai Hospital, Toronto, Ontario</wicri:regionArea>
<wicri:noRegion>Ontario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Oshiro, Elisa E" sort="Oshiro, Elisa E" uniqKey="Oshiro E" first="Elisa E." last="Oshiro">Elisa E. Oshiro</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Ferreira, Rita C C" sort="Ferreira, Rita C C" uniqKey="Ferreira R" first="Rita C. C." last="Ferreira">Rita C. C. Ferreira</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Chigardze, Dimitri Y" sort="Chigardze, Dimitri Y" uniqKey="Chigardze D" first="Dimitri Y." last="Chigardze">Dimitri Y. Chigardze</name>
<affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, University of Cambridge, Cambridge</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Barbosa, Joao Alexandre Goncalves" sort="Barbosa, Joao Alexandre Goncalves" uniqKey="Barbosa J" first="João Alexandre Gonçalves" last="Barbosa">João Alexandre Gonçalves Barbosa</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Departamento de Genética, Universidade Católica de Brasília, Brasilia, Districto Federal, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Genética, Universidade Católica de Brasília, Brasilia, Districto Federal</wicri:regionArea>
<wicri:noRegion>Districto Federal</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="De Souza Ferreira, Luis Carlos" sort="De Souza Ferreira, Luis Carlos" uniqKey="De Souza Ferreira L" first="Luís Carlos" last="De Souza Ferreira">Luís Carlos De Souza Ferreira</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Microbiologia, Universidade de São Paulo, São Paulo, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Benedetti, Celso E" sort="Benedetti, Celso E" uniqKey="Benedetti C" first="Celso E." last="Benedetti">Celso E. Benedetti</name>
<affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Blundell, Tom L" sort="Blundell, Tom L" uniqKey="Blundell T" first="Tom L." last="Blundell">Tom L. Blundell</name>
<affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, University of Cambridge, Cambridge</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Balan, Andrea" sort="Balan, Andrea" uniqKey="Balan A" first="Andrea" last="Balan">Andrea Balan</name>
<affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo, Brazil</addr-line>
</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Laboratório Nacional de Biociências, Centro de Pesquisa em Energia e Materiais, Campinas, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkanesulfonates (chemistry)</term>
<term>Alkanesulfonates (metabolism)</term>
<term>Amino Acid Sequence</term>
<term>Bacterial Proteins (chemistry)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Bacterial Proteins (physiology)</term>
<term>Binding Sites</term>
<term>Carrier Proteins (chemistry)</term>
<term>Carrier Proteins (genetics)</term>
<term>Carrier Proteins (physiology)</term>
<term>Citrus sinensis (growth & development)</term>
<term>Citrus sinensis (metabolism)</term>
<term>Citrus sinensis (microbiology)</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Plant Diseases (microbiology)</term>
<term>Polysaccharides, Bacterial (metabolism)</term>
<term>Sequence Alignment</term>
<term>Virulence</term>
<term>X-Ray Diffraction</term>
<term>Xanthomonas (genetics)</term>
<term>Xanthomonas (metabolism)</term>
<term>Xanthomonas (pathogenicity)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alkanesulfonates</term>
<term>Bacterial Proteins</term>
<term>Carrier Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
<term>Carrier Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkanesulfonates</term>
<term>Polysaccharides, Bacterial</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Bacterial Proteins</term>
<term>Carrier Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Xanthomonas</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Citrus sinensis</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term>
<term>Xanthomonas</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term>
<term>Plant Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Xanthomonas</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Binding Sites</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Sequence Alignment</term>
<term>Virulence</term>
<term>X-Ray Diffraction</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The uptake of sulphur-containing compounds plays a pivotal role in the physiology of bacteria that live in aerobic soils where organosulfur compounds such as sulphonates and sulphate esters represent more than 95% of the available sulphur. Until now, no information has been available on the uptake of sulphonates by bacterial plant pathogens, particularly those of the <italic>Xanthomonas</italic>
 genus, which encompasses several pathogenic species. In the present study, we characterised the alkanesulphonate uptake system (Ssu) of <italic>Xanthomonas axonopodis</italic>
 pv. <italic>citri</italic>
 306 strain (<italic>X. citri</italic>
), the etiological agent of citrus canker.</p>
</sec>
<sec><title>Methodology/Principal Findings</title>
<p>A single operon-like gene cluster (<italic>ssuEDACB</italic>
) that encodes both the sulphur uptake system and enzymes involved in desulphurisation was detected in the genomes of <italic>X. citri</italic>
 and of the closely related species. We characterised <italic>X. citri</italic>
 SsuA protein, a periplasmic alkanesulphonate-binding protein that, together with SsuC and SsuB, defines the alkanesulphonate uptake system. The crystal structure of SsuA bound to MOPS, MES and HEPES, which is herein described for the first time, provides evidence for the importance of a conserved dipole in sulphate group coordination, identifies specific amino acids interacting with the sulphate group and shows the presence of a rather large binding pocket that explains the rather wide range of molecules recognised by the protein. Isolation of an isogenic <italic>ssuA-</italic>
knockout derivative of the <italic>X. citri</italic>
 306 strain showed that disruption of alkanesulphonate uptake affects both xanthan gum production and generation of canker lesions in sweet orange leaves.</p>
</sec>
<sec><title>Conclusions/Significance</title>
<p>The present study unravels unique structural and functional features of the <italic>X. citri</italic>
 SsuA protein and provides the first experimental evidence that an ABC uptake system affects the virulence of this phytopathogen.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Kertesz, Ma" uniqKey="Kertesz M">MA Kertesz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kertesz, Ma" uniqKey="Kertesz M">MA Kertesz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Der, Ploegjr" uniqKey="Van Der P">PloegJR Van der</name>
</author>
<author><name sortKey="Eichorn, E" uniqKey="Eichorn E">E Eichorn</name>
</author>
<author><name sortKey="Leisinger, T" uniqKey="Leisinger T">T Leisinger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Autry, Ar" uniqKey="Autry A">AR Autry</name>
</author>
<author><name sortKey="Fitzgerald, Jw" uniqKey="Fitzgerald J">JW Fitzgerald</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Der, Ploegjr" uniqKey="Van Der P">PloegJR van der</name>
</author>
<author><name sortKey="Weiss, Ma" uniqKey="Weiss M">MA Weiss</name>
</author>
<author><name sortKey="Saller, E" uniqKey="Saller E">E Saller</name>
</author>
<author><name sortKey="Nashimoto, H" uniqKey="Nashimoto H">H Nashimoto</name>
</author>
<author><name sortKey="Saito, N" uniqKey="Saito N">N Saito</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Der, Ploegjr" uniqKey="Van Der P">PloegJR van der</name>
</author>
<author><name sortKey="Cummings, Nj" uniqKey="Cummings N">NJ Cummings</name>
</author>
<author><name sortKey="Leisinger, T" uniqKey="Leisinger T">T Leisinger</name>
</author>
<author><name sortKey="Connerton, If" uniqKey="Connerton I">IF Connerton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Der, Ploegjr" uniqKey="Van Der P">PloegJR Van der</name>
</author>
<author><name sortKey="Iwanicka Nowicka, R" uniqKey="Iwanicka Nowicka R">R Iwanicka-Nowicka</name>
</author>
<author><name sortKey="Bykowski, T" uniqKey="Bykowski T">T Bykowski</name>
</author>
<author><name sortKey="Hryniewicz, Mm" uniqKey="Hryniewicz M">MM Hryniewicz</name>
</author>
<author><name sortKey="Leisinger, T" uniqKey="Leisinger T">T Leisinger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Beale, J" uniqKey="Beale J">J Beale</name>
</author>
<author><name sortKey="Lee, Sy" uniqKey="Lee S">SY Lee</name>
</author>
<author><name sortKey="Iwata, S" uniqKey="Iwata S">S Iwata</name>
</author>
<author><name sortKey="Beis, K" uniqKey="Beis K">K Beis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kahnert, A" uniqKey="Kahnert A">A Kahnert</name>
</author>
<author><name sortKey="Vermeij, P" uniqKey="Vermeij P">P Vermeij</name>
</author>
<author><name sortKey="Wietek, C" uniqKey="Wietek C">C Wietek</name>
</author>
<author><name sortKey="James, P" uniqKey="James P">P James</name>
</author>
<author><name sortKey="Leisinger, T" uniqKey="Leisinger T">T Leisinger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parkinson, N" uniqKey="Parkinson N">N Parkinson</name>
</author>
<author><name sortKey="Aritua, V" uniqKey="Aritua V">V Aritua</name>
</author>
<author><name sortKey="Heeney, J" uniqKey="Heeney J">J Heeney</name>
</author>
<author><name sortKey="Cowie, C" uniqKey="Cowie C">C Cowie</name>
</author>
<author><name sortKey="Bew, J" uniqKey="Bew J">J Bew</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ryan, Rp" uniqKey="Ryan R">RP Ryan</name>
</author>
<author><name sortKey="Vorholter, Fj" uniqKey="Vorholter F">FJ Vorhölter</name>
</author>
<author><name sortKey="Potnis, N" uniqKey="Potnis N">N Potnis</name>
</author>
<author><name sortKey="Jones, Jb" uniqKey="Jones J">JB Jones</name>
</author>
<author><name sortKey="Van Sluys, Ma" uniqKey="Van Sluys M">MA Van Sluys</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gottwald, Tr" uniqKey="Gottwald T">TR Gottwald</name>
</author>
<author><name sortKey="Graham, Jh" uniqKey="Graham J">JH Graham</name>
</author>
<author><name sortKey="Civerolo, El" uniqKey="Civerolo E">EL Civerolo</name>
</author>
<author><name sortKey="Barret, Hc" uniqKey="Barret H">HC Barret</name>
</author>
<author><name sortKey="Hearn, Cj" uniqKey="Hearn C">CJ Hearn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brunings, Am" uniqKey="Brunings A">AM Brunings</name>
</author>
<author><name sortKey="Gabriel, Dw" uniqKey="Gabriel D">DW Gabriel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vojnov, Aa" uniqKey="Vojnov A">AA Vojnov</name>
</author>
<author><name sortKey="Zorreguieta, A" uniqKey="Zorreguieta A">A Zorreguieta</name>
</author>
<author><name sortKey="Dow, Jm" uniqKey="Dow J">JM Dow</name>
</author>
<author><name sortKey="Daniels, Mj" uniqKey="Daniels M">MJ Daniels</name>
</author>
<author><name sortKey="Dankert, M" uniqKey="Dankert M">M Dankert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crossman, L" uniqKey="Crossman L">L Crossman</name>
</author>
<author><name sortKey="Dow, Jm" uniqKey="Dow J">JM Dow</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guo, Y" uniqKey="Guo Y">Y Guo</name>
</author>
<author><name sortKey="Sagaram, Us" uniqKey="Sagaram U">US Sagaram</name>
</author>
<author><name sortKey="Kim, Js" uniqKey="Kim J">JS Kim</name>
</author>
<author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cooke, Am" uniqKey="Cooke A">AM Cooke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Eichhorn, E" uniqKey="Eichhorn E">E Eichhorn</name>
</author>
<author><name sortKey="Van Der, Ploegjr" uniqKey="Van Der P">PloegJR van der</name>
</author>
<author><name sortKey="Leisinger, T" uniqKey="Leisinger T">T Leisinger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, Jj" uniqKey="He J">JJ He</name>
</author>
<author><name sortKey="Quiocho, Fa" uniqKey="Quiocho F">FA Quiocho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name>
</author>
<author><name sortKey="Luecke, H" uniqKey="Luecke H">H Luecke</name>
</author>
<author><name sortKey="Yao, N" uniqKey="Yao N">N Yao</name>
</author>
<author><name sortKey="Quiocho, Fa" uniqKey="Quiocho F">FA Quiocho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Koropatkin, Nm" uniqKey="Koropatkin N">NM Koropatkin</name>
</author>
<author><name sortKey="Pakrasi, Hb" uniqKey="Pakrasi H">HB Pakrasi</name>
</author>
<author><name sortKey="Smith, Tj" uniqKey="Smith T">TJ Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kopriva, S" uniqKey="Kopriva S">S Kopriva</name>
</author>
<author><name sortKey="Koprivova, A" uniqKey="Koprivova A">A Koprivova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Denny, Tp" uniqKey="Denny T">TP Denny</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rigano, La" uniqKey="Rigano L">LA Rigano</name>
</author>
<author><name sortKey="Siciliano, F" uniqKey="Siciliano F">F Siciliano</name>
</author>
<author><name sortKey="Enrique, J" uniqKey="Enrique J">J Enrique</name>
</author>
<author><name sortKey="Sendin, L" uniqKey="Sendin L">L Sendín</name>
</author>
<author><name sortKey="Filippone, P" uniqKey="Filippone P">P Filippone</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kemp, Bp" uniqKey="Kemp B">BP Kemp</name>
</author>
<author><name sortKey="Horne, J" uniqKey="Horne J">J Horne</name>
</author>
<author><name sortKey="Bryant, A" uniqKey="Bryant A">A Bryant</name>
</author>
<author><name sortKey="Cooper, Rm" uniqKey="Cooper R">RM Cooper</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vanderslice, Rw" uniqKey="Vanderslice R">RW Vanderslice</name>
</author>
<author><name sortKey="Doherty, Dh" uniqKey="Doherty D">DH Doherty</name>
</author>
<author><name sortKey="Capage, Ma" uniqKey="Capage M">MA Capage</name>
</author>
<author><name sortKey="Betlach, Mr" uniqKey="Betlach M">MR Betlach</name>
</author>
<author><name sortKey="Hassler, Ra" uniqKey="Hassler R">RA Hassler</name>
</author>
<author><name sortKey="Crescenzi, V" uniqKey="Crescenzi V">V Crescenzi</name>
</author>
<author><name sortKey="Dea, Icm" uniqKey="Dea I">ICM Dea</name>
</author>
<author><name sortKey="Paoletti, S" uniqKey="Paoletti S">S Paoletti</name>
</author>
<author><name sortKey="Stivala, Ss" uniqKey="Stivala S">SS Stivala</name>
</author>
<author><name sortKey="Sutherland, Iw" uniqKey="Sutherland I">IW Sutherland</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Thompson, Dg" uniqKey="Thompson D">DG Thompson</name>
</author>
<author><name sortKey="Higgins, Jd" uniqKey="Higgins J">JD Higgins</name>
</author>
<author><name sortKey="Gibson, Tj" uniqKey="Gibson T">TJ Gibson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Saitou, N" uniqKey="Saitou N">N Saitou</name>
</author>
<author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name>
</author>
<author><name sortKey="Peterson, D" uniqKey="Peterson D">D Peterson</name>
</author>
<author><name sortKey="Peterson, N" uniqKey="Peterson N">N Peterson</name>
</author>
<author><name sortKey="Stecher, G" uniqKey="Stecher G">G Stecher</name>
</author>
<author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Edelhoch, H" uniqKey="Edelhoch H">H Edelhoch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Deleage, G" uniqKey="Deleage G">G Deleage</name>
</author>
<author><name sortKey="Geourjon, C" uniqKey="Geourjon C">C Geourjon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Polikarpov, I" uniqKey="Polikarpov I">I Polikarpov</name>
</author>
<author><name sortKey="Oliva, G" uniqKey="Oliva G">G Oliva</name>
</author>
<author><name sortKey="Castellano, Ee" uniqKey="Castellano E">EE Castellano</name>
</author>
<author><name sortKey="Garratt, Rc" uniqKey="Garratt R">RC Garratt</name>
</author>
<author><name sortKey="Arruda, P" uniqKey="Arruda P">P Arruda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kabsch, W" uniqKey="Kabsch W">W Kabsch</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Vonrhein, C" uniqKey="Vonrhein C">C Vonrhein</name>
</author>
<author><name sortKey="Blanc, E" uniqKey="Blanc E">E Blanc</name>
</author>
<author><name sortKey="Roversi, P" uniqKey="Roversi P">P Roversi</name>
</author>
<author><name sortKey="Bricogne, G" uniqKey="Bricogne G">G Bricogne</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sheldrick, Gm" uniqKey="Sheldrick G">GM Sheldrick</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Murshudov, Gn" uniqKey="Murshudov G">GN Murshudov</name>
</author>
<author><name sortKey="Vagin, Aa" uniqKey="Vagin A">AA Vagin</name>
</author>
<author><name sortKey="Dodson, Ej" uniqKey="Dodson E">EJ Dodson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oshiro, Ee" uniqKey="Oshiro E">EE Oshiro</name>
</author>
<author><name sortKey="Nepomuceno, Rsl" uniqKey="Nepomuceno R">RSL Nepomuceno</name>
</author>
<author><name sortKey="Faria, Jb" uniqKey="Faria J">JB Faria</name>
</author>
<author><name sortKey="Ferreira, Lcs" uniqKey="Ferreira L">LCS Ferreira</name>
</author>
<author><name sortKey="Ferreira, Rcc" uniqKey="Ferreira R">RCC Ferreira</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Baldini, Rl" uniqKey="Baldini R">RL Baldini</name>
</author>
<author><name sortKey="Tahara, St" uniqKey="Tahara S">ST Tahara</name>
</author>
<author><name sortKey="Rosato, Yb" uniqKey="Rosato Y">YB Rosato</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cernadas, Ra" uniqKey="Cernadas R">RA Cernadas</name>
</author>
<author><name sortKey="Camillo, Lr" uniqKey="Camillo L">LR Camillo</name>
</author>
<author><name sortKey="Benedetti, Ce" uniqKey="Benedetti C">CE Benedetti</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Brésil</li>
<li>Canada</li>
<li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Angleterre de l'Est</li>
<li>État de São Paulo</li>
</region>
<settlement><li>Cambridge</li>
<li>São Paulo</li>
</settlement>
<orgName><li>Université de Cambridge</li>
<li>Université de São Paulo</li>
</orgName>
</list>
<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="T Foli De Araujo, Fabiano" sort="T Foli De Araujo, Fabiano" uniqKey="T Foli De Araujo F" first="Fabiano" last="T Foli De Araújo">Fabiano T Foli De Araújo</name>
</region>
<name sortKey="Balan, Andrea" sort="Balan, Andrea" uniqKey="Balan A" first="Andrea" last="Balan">Andrea Balan</name>
<name sortKey="Barbosa, Joao Alexandre Goncalves" sort="Barbosa, Joao Alexandre Goncalves" uniqKey="Barbosa J" first="João Alexandre Gonçalves" last="Barbosa">João Alexandre Gonçalves Barbosa</name>
<name sortKey="Benedetti, Celso E" sort="Benedetti, Celso E" uniqKey="Benedetti C" first="Celso E." last="Benedetti">Celso E. Benedetti</name>
<name sortKey="De Souza Ferreira, Luis Carlos" sort="De Souza Ferreira, Luis Carlos" uniqKey="De Souza Ferreira L" first="Luís Carlos" last="De Souza Ferreira">Luís Carlos De Souza Ferreira</name>
<name sortKey="Ferreira, Rita C C" sort="Ferreira, Rita C C" uniqKey="Ferreira R" first="Rita C. C." last="Ferreira">Rita C. C. Ferreira</name>
<name sortKey="Oshiro, Elisa E" sort="Oshiro, Elisa E" uniqKey="Oshiro E" first="Elisa E." last="Oshiro">Elisa E. Oshiro</name>
<name sortKey="Pereira, Cristiane T" sort="Pereira, Cristiane T" uniqKey="Pereira C" first="Cristiane T." last="Pereira">Cristiane T. Pereira</name>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Bolanos Garcia, Victor M" sort="Bolanos Garcia, Victor M" uniqKey="Bolanos Garcia V" first="Victor M." last="Bolanos-Garcia">Victor M. Bolanos-Garcia</name>
</region>
<name sortKey="Blundell, Tom L" sort="Blundell, Tom L" uniqKey="Blundell T" first="Tom L." last="Blundell">Tom L. Blundell</name>
<name sortKey="Chigardze, Dimitri Y" sort="Chigardze, Dimitri Y" uniqKey="Chigardze D" first="Dimitri Y." last="Chigardze">Dimitri Y. Chigardze</name>
</country>
<country name="Canada"><noRegion><name sortKey="Sanches, Mario" sort="Sanches, Mario" uniqKey="Sanches M" first="Mario" last="Sanches">Mario Sanches</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D19 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000D19 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3839906
   |texte=   Structural and Physiological Analyses of the Alkanesulphonate-Binding Protein (SsuA) of the Citrus Pathogen Xanthomonas citri
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:24282519" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a OrangerV1

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

Serveur d'exploration sur l'oranger

Structural and Physiological Analyses of the Alkanesulphonate-Binding Protein (SsuA) of the Citrus Pathogen Xanthomonas citri

Structural and Physiological Analyses of the Alkanesulphonate-Binding Protein (SsuA) of the Citrus Pathogen Xanthomonas citri

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.